Pyruvate Kinase antibody

Catalog No. ABIN106389

Product Details anti-Pyruvate Kinase Antibody

Target: Pyruvate Kinase (PK)

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Pyruvate Kinase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.

Purity: Anti-PYRUVATE KINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle). This product has been reported to react with all forms of pyruvate kinase (pan M-PK).

Endotoxin Level: Low Endotoxin : No

Immunogen: Pyruvate Kinase (Rabbit Muscle)
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: ELISA Dilution: 1:4.000 - 1:20.000
Immunohistochemistry Dilution: 1:500 - 1:2.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Alternative Name: PYRUVATE KINASE (PK Products)

Background: Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody

Gene ID: 100008676

NCBI Accession: NP_001182574

UniProt: P11974