PKM antibody

Catalog No. ABIN106387

Supplier Product No.: 100-1178

Product Details anti-PKM Antibody

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PKM antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Supplier Product No.: 100-1178

Supplier: Rockland

Purpose: Pyruvate Kinase Antibody

Cross-Reactivity (Details): This product has been reported to react with all forms of pyruvate kinase (pan M-PK).

Characteristics: Synonyms: goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2

Purification: Anti-PYRUVATE KINASE Antibody immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Pyruvate Kinase [Rabbit Muscle].

Immunogen:

Immunogen: Pyruvate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-PYRUVATE KINASE Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Specific conditions should be optimized by user.

Western Blot Dilution: 1:5,000 - 1:20,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:50,000 - 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 40 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-PKM antibody (ABIN106387)

Shimada, Shinagawa, Ishii: "Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 3, pp. 245-54, (2008) (PubMed).

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM (PKM Products)

Background: Background: Pyruvate kinase (EC: 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a magnesium ion. The enzyme is a 'transferase' under the international classification of enzymes. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. However, in anaerobic glycolysis, lactate dehydrogenase will utilize the NADH produced by glyceraldehyde phosphate dehydrogenase to reduce pyruvate to lactate. In humans, there are two pyruvate kinase isozymes: type M (muscle, SwissProt P14618) and type L,R (liver and erythrocyte, SwissProt P30613). The isozymes differ in primary structure and regulation. This reaction has a large positive free energy change, one of three in glycolysis. All three such steps regulate the overall activity of the pathway, and are, in general, irreversible under wild-type conditions. Anti-Pyruvate kinase antibody is idea for investigators involved in cytokines and growth factor research.

Gene ID: 100008676

NCBI Accession: NP_001182574

UniProt: P11974

Pathways: Warburg Effect