Protein G antibody

Catalog No. ABIN106214

Supplier Product No.: 200-401-138-0100

Product Details anti-Protein G Antibody

Target: Protein G

Reactivity: Streptococcus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Protein G antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 200-401-138-0100

Supplier: Rockland

Purpose: Protein G Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Protein G.

Characteristics: Synonyms: rabbit anti-Protein G Antibody, Rabbit anti Protein G, Rabbit anti PG

Purification: Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Protein G [Streptococcus species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Protein G is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Protein G

Target: Protein G

Abstract: Protein G Products

Background: Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.