PPARD antibody (N-Term)

Catalog No. ABIN105799

Supplier Product No.: 600-401-420

Product Details anti-PPARD Antibody

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-420

Supplier: Rockland

Purpose: PPAR delta Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse PPAR delta protein.

Characteristics: Synonyms: rabbit anti-PPAR delta antibody, PPARD, PPARB, NR1C2, PPAR-delta, NUCI, Nuclear hormone receptor 1 antibody, Nuclear receptor subfamily 1 group C member 2 antibody, Nuclear hormone receptor 1, NUC-1, Peroxisome proliferative activated receptor delta antibody, Peroxisome proliferator-activated receptor beta, PPAR-beta

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-PPAR delta antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500

Application Note: This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 43 kDa in size corresponding to PPAR delta by western blot in the appropriate tissue or cell lysate.

Western Blot Dilution: 1:500 - 1:5,000

ELISA Dilution: 1:30,000 - 1:70,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.57 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-PPARD antibody (ABIN105799)

Matsushita, Hassanein, Martinez-Clemente, Lazaro, French, Xie, Lai, Karin, Tsukamoto: "Gender difference in NASH susceptibility: Roles of hepatocyte Ikkβ and Sult1e1." in: PLoS ONE, Vol. 12, Issue 8, pp. e0181052, (2017) (PubMed).

Target Details for PPARD

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Alternative Name: Ppard (PPARD Products)

Background: Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones.  Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Gene ID: 19015, 548577

UniProt: P35396

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation