Peroxidase antibody

Catalog No. ABIN105518

Supplier Product No.: 200-6138-0100

Product Details anti-Peroxidase Antibody

Target: Peroxidase

Reactivity: Horseradish

Host: Sheep

Clonality: Polyclonal

Conjugate: This Peroxidase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-6138-0100

Supplier: Rockland

Cross-Reactivity (Details): Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-PEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Peroxidase [Horseradish].

Endotoxin Level: Low Endotoxin : No

Immunogen: Peroxidase [Horseradish]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
ELISA Dilution: 1:2.000 - 1:10.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Peroxidase

Target: Peroxidase

Abstract: Peroxidase Products