Pepsin A antibody

Catalog No. ABIN105444

Product Details anti-Pepsin A Antibody

Target: Pepsin A

Reactivity: Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Pepsin A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: Pepsin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].

Characteristics: Synonyms: goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody

Purification: Anti-PEPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:2,000 - 1:10,000

Application Note: Anti-Pepsin antibody has been tested in ELISA, western blot and immunohistochemistry. This product detects a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:5,000 - 1:20,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:50:000 - 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Pepsin A

Target: Pepsin A

Alternative Name: PGA (Pepsin A Products)

Background: Background: Pepsin antibody detects Pepsin protein. Pepsin is an enzyme whose pepsinogen is released by the chief cells in the stomach and degrades food proteins into peptides. Pepsin belongs to the peptidase A1 family and is one of three principal proteolytic enzymes in the digestive system. Pepsin is most efficient in cleaving peptide bonds between hydrophobic and preferably aromatic amino acids such as phenylalanine, tryptophan, and tyrosine at positions P1 and P1'. Anti-Pepsin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.

Gene ID: 396892

UniProt: P00791