p53 antibody

Catalog No. ABIN105263

Supplier Product No.: 200-301-174

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-174

Supplier: Rockland

Purpose: p53 Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53.

Characteristics: Synonyms: mouse anti-p53 antibody, mouse anti-Tumor Suppressor p53 antibody, Phosphoprotein p53 antibody, TP53 antibody, Transformation related protein 53 antibody, TRP53 antibody, cellular Tumor antigen p53 antibody

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.

Immunogen Type: Recombinant Protein

Clone: BP53-12

Isotype: IgG2a kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-p53 has been tested by western blot and immunofluorescence and is suitable for ChIP, flow cytometry, immunohistochemistry, Immunofluorescence, immunoblotting and immunoprecipitation.  p53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70 % but incidence varies from zero in carcinoid lung tumors to 97 % in primary melanomas.  High concentrations of p53 protein are transiently expressed in human epidermis and superficial dental fibroblasts following mild ultraviolet irradiation. This antibody reacts with an N-terminal epitope of the 53 kD gene product and this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwaving is needed for optimal staining.

ChIP Dilution: 1 μg/μL at 4° o/n

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-p53 antibody (ABIN105263)

Sikorski, Mehta, Inngjerdingen, Thakor, Kling, Kalina, Nyman, Stensland, Zhou, de Souza, Holden, Stuchly, Templin, Lund-Johansen: "A high-throughput pipeline for validation of antibodies." in: Nature methods, Vol. 15, Issue 11, pp. 909-912, (2019) (PubMed).

Lane: "Cancer. p53, guardian of the genome." in: Nature, Vol. 358, Issue 6381, pp. 15-6, (1992) (PubMed).

Hollstein, Sidransky, Vogelstein, Harris: "p53 mutations in human cancers." in: Science (New York, N.Y.), Vol. 253, Issue 5015, pp. 49-53, (1991) (PubMed).

Gurney, Harrison, Fenno: "Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens." in: Journal of virology, Vol. 34, Issue 3, pp. 752-63, (1980) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: TP53 (TP53 Products)

Background: Background: The p53 gene like the Rb gene, is a tumor suppressor gene. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism, its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are pre-disposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. Anti-p53 Antibody is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, and p38 Pathway research.

Gene ID: 7157, 23491729

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect