PAK1/2/3 antibody (pThr423)

Catalog No. ABIN105217

Product Details anti-PAK1/2/3 Antibody

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Binding Specificity: pThr423

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1/2/3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: This phospho specific polyclonal antibody was tested by ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.   Although not tested, this antibody is likely functional in immunohistochemistry, immunoblotting, and immuno-precipitation.  Lysates from Jurkat cells or PAK transfected cells may be used as a control.This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.94 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK 1/2/3 (PAK1/2/3 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, pak1.S antibody, PAK1 antibody

Background: The p21-activated kinases (PAKs) are a family of multifunctional serine/threonine kinases involved in a variety of cell functions including stress response, apoptosis and regulation of cell motility and tumor metastasis.  Mammalian PAKs are called 1, 2, 3 or a, g, b respectively.  PAKs are part of a large family of kinases where the catalytic domain of the kinase is related to Ste20 kinase of S. cerevisiae.  Pak activity is regulated by specific binding of GTP-bound Rac and cdc42 GTPases and also by sphingosine and related lipids.  PAK1 activation is induced by a variety of growth factors and G-protein-coupled receptors, Fc receptors, and integrins.  This antibody is specific for the phosphorylated form of PAK 1/2/3.  The selected peptide sequence used to generate the polyclonal antibody is common to all human PAKs.
Synonyms: Alpha PAK antibody, Gamma PAK antibody, Beta PAK antibody

Gene ID: 5058

UniProt: Q13153