Ovalbumin antibody (HRP)

Catalog No. ABIN105184

Supplier Product No.: 200-4333-0100

Product Details anti-Ovalbumin Antibody (HRP)

Target: Ovalbumin (OVA)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ovalbumin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Supplier Product No.: 200-4333-0100

Supplier: Rockland

Purpose: Ovalbumin Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

Characteristics: Synonyms: rabbit anti-Ovalbumin Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White

Purification: Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Ovalbumin Antibody was produced by repeated immunizations with hen egg white Ovalbumin protein.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Ovalbumin Antibody Peroxidase Conjugated has been tested by ELISA and western blot and is suitable for IHC and IP. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-Ovalbumin antibody (HRP) (ABIN105184)

Apperson, Vorachek, Dolan, Bobe, Pirelli, Hall: "Effects of feeding pregnant beef cows selenium-enriched alfalfa hay on passive transfer of ovalbumin in their newborn calves." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 50, pp. 640-645, (2019) (PubMed).

Target Details for Ovalbumin

Target: Ovalbumin (OVA)

Alternative Name: SERPINB14 (OVA Products)

Background: Background: Anti-Ovalbumin Antibody detects Ovalbumin. Ovalbumin is the main protein found in egg white, making up 60-65 % of the total protein. It is a non-inhibitory serpin. It is the storage protein of egg white. Anti-Ovalbumin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Gene ID: 396058

UniProt: P01012