Ovalbumin antibody

Catalog No. ABIN105182

Supplier Product No.: 200-401-033

Product Details anti-Ovalbumin Antibody

Target: Ovalbumin (OVA)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ovalbumin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Supplier Product No.: 200-401-033

Supplier: Rockland

Purpose: Ovalbumin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

Characteristics: Synonyms: rabbit anti-Ovalbumin Antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White

Purification: Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Ovalbumin [Hen Egg White]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:300 - 1:2,000

Application Note: Anti-Ovalbumin Antibody has been tested in Western Blot. Although not tested this product could be useful in assays such as ELISA, IHC and IP. Specific conditions for reactivity and signal detection should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Ovalbumin antibody (ABIN105182)

Apperson, Vorachek, Dolan, Bobe, Pirelli, Hall: "Effects of feeding pregnant beef cows selenium-enriched alfalfa hay on passive transfer of ovalbumin in their newborn calves." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 50, pp. 640-645, (2019) (PubMed).

Target Details for Ovalbumin

Target: Ovalbumin (OVA)

Alternative Name: SERPINB14 (OVA Products)

Background: Background: Anti-Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 55-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins, it does not undergo a conformational change upon proteolytic cleavage and is not a serine protease inhibitor. The function of ovalbumin is still unknown, although it is presumed to be a storage protein for tissue-specific, steroid hormone-induced gene expression. Ovalbumin is a key protein for biochemical investigations and processes and can be used as a control, blocking reagent, or antibody in assays such as ELISA, SDS-PAGE, IHC, Western Blot, and size exclusion chromatography.

Gene ID: 396058

UniProt: P01012