UNC5B antibody (Internal Region)

Catalog No. ABIN1049514

Product Details anti-UNC5B Antibody

Target: UNC5B (Netrin receptor UNC5B (UNC5B))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UNC5B antibody is un-conjugated

Application: ELISA

Purpose: Unc5b (mouse, rat)

Sequence: DWIFQLKTQA HQGH

Specificity: This antibody is expected to recognize the cytoplasmic domain of the protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Recent

Immunogen: Peptide with sequence C-DWIFQLKTQAHQGH, from the of the protein sequence according to NP_084046.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments in Human and rodent Brain, Lung and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results be

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-UNC5B antibody (ABIN1049514)

Choi, de Poot, Lee, Kim, Han, Kim, Finley, Lee: "Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation." in: Nature communications, Vol. 7, pp. 10963, (2016) (PubMed).

Target Details for UNC5B

Target: UNC5B (Netrin receptor UNC5B (UNC5B))

Alternative Name: Unc5b (UNC5B Products)

Synonyms: unc5h2 antibody, 6330415E02Rik antibody, A630020F16 antibody, D10Bwg0792e antibody, Unc5h2 antibody, UNC5H2 antibody, p53RDL1 antibody, UNC5C antibody, UNC5B antibody, p53rdl1 antibody, unc-5 netrin receptor B antibody, unc-5 netrin receptor B L homeolog antibody, unc5b antibody, Unc5b antibody, UNC5B antibody, unc5b.L antibody

Background: Unc5b, unc-5 homolog B (C. elegans), 6330415E02Rik, A630020F16, D10Bwg0792e, Unc5h2, netrin receptor UNC5B, protein unc-5 homolog 2, protein unc-5 homolog B, unc-5 homolog 2, unc5 homolog 2

Gene ID: 219699, 107449, 60630

NCBI Accession: NP_084046