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NCKAP1 antibody (Internal Region)

Verified NCKAP1 Reactivity: Human ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN1049510
  • Target See all NCKAP1 Antibodies
    NCKAP1 (NCK Associated Protein 1 (NCKAP1))
    Binding Specificity
    • 15
    • 7
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 32
    • 27
    • 11
    Human
    Host
    • 47
    Goat
    Clonality
    • 47
    Polyclonal
    Conjugate
    • 16
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NCKAP1 antibody is un-conjugated
    Application
    • 37
    • 17
    • 3
    • 2
    • 2
    • 2
    • 1
    ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    NCKAP1
    Sequence
    TPREYLTSHL EIR
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_038464.1, NP_995314.1).
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-TPREYLTSHLEIR, from the of the protein sequence according to NP_038464.1, NP_995314.1.
    Isotype
    IgG
    Top Product
    Discover our top product NCKAP1 Primary Antibody
  • Application Notes

    Western Blot: Preliminary experiments gave an approx 100 kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5 μg/mL antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calcu

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    NCKAP1 (NCK Associated Protein 1 (NCKAP1))
    Alternative Name
    NCKAP1 (NCKAP1 Products)
    Synonyms
    HEM2 antibody, NAP1 antibody, NAP125 antibody, p125Nap1 antibody, C79304 antibody, H19 antibody, Hem-2 antibody, Hem2 antibody, Nap1 antibody, mKIAA0587 antibody, mh19 antibody, NCK associated protein 1 antibody, NCK-associated protein 1 antibody, NCK-associated protein 1 L homeolog antibody, NCKAP1 antibody, Nckap1 antibody, nckap1.L antibody
    Background
    NCKAP1, NCK-associated protein 1, FLJ11291, HEM2, KIAA0587, MGC8981, NAP1, NAP125, NAP 1, OTTHUMP00000163460, OTTHUMP00000163461, membrane-associated protein HEM-2, nck-associated protein 1, p125Nap1
    Gene ID
    10787, 50884, 58823
    NCBI Accession
    NP_038464, NP_995314
    Pathways
    RTK Signaling, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
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