VIPR2 antibody (Internal Region)

Catalog No. ABIN1049457

Product Details anti-VIPR2 Antibody

Target: VIPR2 (Vasoactive Intestinal Peptide Receptor 2 (VIPR2))

Binding Specificity: Internal Region

Reactivity: Human, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VIPR2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human VIPR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine (100%) Rat, Pig (94%) Mouse, Dog, Rabbit, Opossum (88%) Elephant (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from internal region of human VIPR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine (100%), Rat, Pig (94%), Mouse, Dog, Rabbit, Opossum (88%), Elephant (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for VIPR2

Target: VIPR2 (Vasoactive Intestinal Peptide Receptor 2 (VIPR2))

Alternative Name: VPAC2 / VIPR2 (VIPR2 Products)

Synonyms: C16DUPq36.3 antibody, DUP7q36.3 antibody, PACAP-R-3 antibody, PACAP-R3 antibody, VIP-R-2 antibody, VPAC2 antibody, VPAC2R antibody, VPCAP2R antibody, VIPR2 antibody, LOC100136266 antibody, Vip2 antibody, pacap antibody, vpac2 antibody, vpac2-R antibody, vpac2-r antibody, vpcap2r antibody, LOC100229999 antibody, vasoactive intestinal peptide receptor 2 antibody, vasoactive intestinal polypeptide receptor type 2 antibody, VIPR2 antibody, LOC100136266 antibody, Vipr2 antibody, vipr2 antibody

Background: Name/Gene ID: VIPR2
Subfamily: Vasoactive intestinal polypeptide
Family: GPCR

Synonyms: VIPR2, C16DUPq36.3, DUP7q36.3, PACAP-R-3, Pvr3, Vip type-2 receptor, Vip-r2, Vip2 receptor, PACAP type III receptor, Pacap type-3, PACAP-R3, PACAPR-3, VIP and PACAP receptor 2, VIP-R-2, VIP2R, VPAC2, VPCAP2R, PACAP3, Vip2, VPAC2R

Gene ID: 7434

Pathways: cAMP Metabolic Process