STX1A antibody (N-Term)

Catalog No. ABIN1049369

Product Details anti-STX1A Antibody

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Monkey, Pig, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX1A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human STX1A / Syntaxin 1A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except STX2 (67 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%) Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%) Elephant (87%) Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from N-Terminus of human STX1A / Syntaxin 1A. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%), Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%), Elephant (87%), Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for STX1A

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Alternative Name: STX1A / Syntaxin 1A (STX1A Products)

Synonyms: HPC-1 antibody, P35-1 antibody, STX1 antibody, SYN1A antibody, STX1A antibody, hpc-1 antibody, p35-1 antibody, stx1 antibody, sx1a antibody, syntaxin1a antibody, CG10716 antibody, CG18615 antibody, CG31136 antibody, CG5448 antibody, CT30033 antibody, Dm Syx1 antibody, DmSyx1A antibody, Dmel\\CG31136 antibody, SYX1A antibody, Syt1A antibody, Syx antibody, Syx-1A antibody, Syx1 antibody, Syx1a antibody, SyxA antibody, anon-EST:Gibbs4 antibody, anon-WO02059370.54 antibody, dSyx1 antibody, l(3)06737 antibody, synt antibody, syt-1A antibody, syx antibody, syx-1 antibody, syx-1A antibody, syx1 antibody, syx1A antibody, syntaxin 1A antibody, syntaxin 1A (brain) antibody, Syntaxin 1A antibody, syntaxin-1A antibody, STX1A antibody, Stx1a antibody, stx1a antibody, Syx1A antibody, EDI_005240 antibody, Tsp_07354 antibody

Background: Name/Gene ID: STX1A
Family: Syntaxin/epimorphin

Synonyms: STX1A, Syntaxin-1A, p35-1, SYN1a, Syntaxin 1A, Syntaxin 1A (brain), HPC-1, Neuron-specific antigen HPC-1, STX1

Gene ID: 6804

Pathways: Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport