SLC39A14 antibody (Internal Region)
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- Target See all SLC39A14 Antibodies
- SLC39A14 (Solute Carrier Family 39 (Zinc Transporter), Member 14 (SLC39A14))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Pig, Bat, Dog, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SLC39A14 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human SLC39A14. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Gorilla, Panda, Dog, Bat, Horse, Pig (100%) Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Bovine (94%) Elephant (88%) Rat, Rabbit (81%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 16 amino acid peptide from internal region of human SLC39A14. Percent identity with other species by BLAST analysis: Human, Gorilla, Panda, Dog, Bat, Horse, Pig (100%), Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Bovine (94%), Elephant (88%), Rat, Rabbit (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SLC39A14 Primary Antibody
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- Application Notes
- Approved: IHC, IHC-P (15 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SLC39A14 (Solute Carrier Family 39 (Zinc Transporter), Member 14 (SLC39A14))
- Alternative Name
- SLC39A14 / ZIP14 (SLC39A14 Products)
- Synonyms
- LZT-Hs4 antibody, NET34 antibody, ZIP14 antibody, cig19 antibody, BC021530 antibody, G630015O18Rik antibody, Zip14 antibody, fad123 antibody, solute carrier family 39 member 14 antibody, solute carrier family 39 (zinc transporter), member 14 antibody, SLC39A14 antibody, Slc39a14 antibody
- Background
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Name/Gene ID: SLC39A14
Subfamily: Zinc-iron transporter
Family: Transporter
Synonyms: SLC39A14, KIAA0062, LZT-Hs4, NET34, Zrt-, Irt-like protein 14, Zinc transporter ZIP14, ZIP-14, ZIP14, Zrt- and Irt-like protein 14, Cig19 - Gene ID
- 23516
- Pathways
- Transition Metal Ion Homeostasis
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