RXFP2 antibody (Extracellular Domain)

Catalog No. ABIN1049307

Product Details anti-RXFP2 Antibody

Target: RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))

Binding Specificity: Extracellular Domain

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RXFP2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RXFP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RXFP1 (53 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Marmoset, Elephant, Panda, Bat, Horse (94%) Monkey, Dog, Pig (88%) Bovine, Rabbit, Platypus, Xenopus (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from 2nd extracellular domain of human RXFP2. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Marmoset, Elephant, Panda, Bat, Horse (94%), Monkey, Dog, Pig (88%), Bovine, Rabbit, Platypus, Xenopus (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (18 - 72 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RXFP2

Target: RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))

Alternative Name: RXFP2 / LGR8 (RXFP2 Products)

Synonyms: GPR106 antibody, GREAT antibody, INSL3R antibody, LGR8 antibody, LGR8.1 antibody, RXFPR2 antibody, Gpcr antibody, Lgr8 antibody, Gpr106 antibody, Great antibody, lgr8.1 antibody, rxfp2 antibody, rxfp2b antibody, zgc:63668 antibody, relaxin/insulin like family peptide receptor 2 antibody, relaxin/insulin-like family peptide receptor 2 antibody, relaxin/insulin-like family peptide receptor 2a antibody, RXFP2 antibody, Rxfp2 antibody, rxfp2a antibody

Background: Name/Gene ID: RXFP2
Subfamily: Relaxin
Family: GPCR

Synonyms: RXFP2, G-protein coupled receptor 106, GPR106, INSL3R, LGR8, GREAT, Relaxin receptor 2, LGR8.1, RXFPR2

Gene ID: 122042

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction