REEP5 antibody (N-Term)

Catalog No. ABIN1049287

Product Details anti-REEP5 Antibody

Target: REEP5 (Receptor Accessory Protein 5 (REEP5))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REEP5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human REEP5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat (100%) Chicken (93%) Elephant, Platypus (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 14 amino acid peptide from N-Terminus of human REEP5. Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat (100%), Chicken (93%), Elephant, Platypus (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for REEP5

Target: REEP5 (Receptor Accessory Protein 5 (REEP5))

Alternative Name: REEP5 / DP1 (REEP5 Products)

Synonyms: fb02g04 antibody, zgc:73197 antibody, wu:fb02g04 antibody, reep6 antibody, MGC130879 antibody, C5orf18 antibody, D5S346 antibody, DP1 antibody, TB2 antibody, YOP1 antibody, AI324241 antibody, AU022809 antibody, AW495741 antibody, DP1/TB2 antibody, Dp1 antibody, R74856 antibody, TB2/DP1 antibody, receptor accessory protein 5 antibody, receptor accessory protein 5 L homeolog antibody, reep5 antibody, REEP5 antibody, reep5.L antibody, Reep5 antibody

Background: Name/Gene ID: REEP5

Synonyms: REEP5, C5orf18, D5S346, Deleted in polyposis 1, Deleted in polyposis, Polyposis locus protein 1, Protein TB2, Receptor accessory protein 5, TB2, YOP1, DP1

Gene ID: 7905

Pathways: SARS-CoV-2 Protein Interactome