PRLHR antibody (Extracellular Domain)

Catalog No. ABIN1049241

Product Details anti-PRLHR Antibody

Target: PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))

Binding Specificity: Extracellular Domain

Reactivity: Human, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRLHR antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human PRLHR / GPR10. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Rat (100%) Mouse, Dog, Bovine, Pig (95%) Panda (91%) Horse (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 22 amino acid peptide from 2nd extracellular domain of human PRLHR / GPR10. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Rat (100%), Mouse, Dog, Bovine, Pig (95%), Panda (91%), Horse (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (1 - 2 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRLHR

Target: PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))

Alternative Name: PRLHR / GPR10 (PRLHR Products)

Synonyms: GPR10 antibody, PRLHR3 antibody, PrRPR antibody, GR3 antibody, Gm339 antibody, Gpr10 antibody, Gr3 antibody, Prrpr antibody, Uhr-1 antibody, prolactin releasing hormone receptor antibody, PRLHR antibody, prlhr2 antibody, Prlhr antibody

Background: Name/Gene ID: PRLHR
Subfamily: Releasing hormone
Family: GPCR

Synonyms: PRLHR, G protein-coupled receptor 10, G-protein coupled receptor 10, GPR10, GR3, HGR3, PrRP receptor, PrRPR, Uhr-1

Gene ID: 2834

Pathways: Feeding Behaviour