P2RY10 antibody (C-Term)

Catalog No. ABIN1049203

Product Details anti-P2RY10 Antibody

Target: P2RY10 (Purinergic Receptor P2Y, G-Protein Coupled, 10 (P2RY10))

Binding Specificity: C-Term

Reactivity: Human, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RY10 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human P2RY10 / P2Y10. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Hamster (100%) Gibbon, Mouse, Rat, Panda, Bat, Bovine, Rabbit, Horse (94%) Marmoset, Elephant, Dog, Opossum (89%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from C-terminus of human P2RY10 / P2Y10. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Hamster (100%), Gibbon, Mouse, Rat, Panda, Bat, Bovine, Rabbit, Horse (94%), Marmoset, Elephant, Dog, Opossum (89%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (6 - 32 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for P2RY10

Target: P2RY10 (Purinergic Receptor P2Y, G-Protein Coupled, 10 (P2RY10))

Alternative Name: P2Y10 / P2RY10 (P2RY10 Products)

Synonyms: 5830408N17Rik antibody, P2Y10 antibody, RGD1561912 antibody, purinergic receptor P2Y, G-protein coupled, 10 antibody, P2Y receptor family member 10 antibody, purinergic receptor P2Y, G-protein coupled, 10 L homeolog antibody, putative P2Y purinoceptor 10 antibody, purinergic receptor P2Y, G-protein coupled 10 antibody, purinergic receptor P2Y10 antibody, P2RY10 antibody, p2ry10.L antibody, LOC100396399 antibody, P2ry10 antibody

Background: Name/Gene ID: P2RY10
Subfamily: Purinergic
Family: GPCR

Synonyms: P2RY10, p2RY10, p2Y-like receptor, Putative P2Y purinoceptor 10, p2Y purinoceptor 10, p2Y10

Gene ID: 27334