NPFFR1 antibody (Extracellular Domain)

Catalog No. ABIN1049114

Product Details anti-NPFFR1 Antibody

Target: NPFFR1 (Neuropeptide FF Receptor 1 (NPFFR1))

Binding Specificity: Extracellular Domain

Reactivity: Human, Cow, Horse, Monkey, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPFFR1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NPFF1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Horse, Rabbit, Pig (100%) Mouse, Rat, Dog, Panda (95%) Elephant, Turkey, Chicken (85%) Opossum, Platypus (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from 2nd extracellular domain of human NPFF1 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Horse, Rabbit, Pig (100%), Mouse, Rat, Dog, Panda (95%), Elephant, Turkey, Chicken (85%), Opossum, Platypus (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (19 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NPFFR1

Target: NPFFR1 (Neuropeptide FF Receptor 1 (NPFFR1))

Alternative Name: NPFFR1 / GPR147 (NPFFR1 Products)

Synonyms: GPR147 antibody, NPFF1 antibody, NPFF1R1 antibody, OT7T022 antibody, Gm236 antibody, Gpr147 antibody, RFRPR antibody, MGC83178 antibody, NPFFR1 antibody, LOC100230250 antibody, neuropeptide FF receptor 1 antibody, neuropeptide FF receptor 1 L homeolog antibody, inorganic pyrophosphatase-like antibody, NPFFR1 antibody, Npffr1 antibody, npffr1.L antibody, LOC100230250 antibody

Background: Name/Gene ID: NPFFR1
Subfamily: Neuropeptide
Family: GPCR

Synonyms: NPFFR1, G-protein coupled receptor 147, GPR147, Neuropeptide FF 1, Neuropeptide FF receptor 1, NPFF-R1, OT7T022, NPFF1 receptor, G protein-coupled receptor 147, Neuropeptide FF1 receptor, NPFF1, NPFF1R1

Gene ID: 64106

Pathways: cAMP Metabolic Process