NEK6 antibody (N-Term)

Catalog No. ABIN1049089

Product Details anti-NEK6 Antibody

Target: NEK6 (NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6))

Binding Specificity: N-Term

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NEK6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 14 amino acid peptide from N-terminus of human NEK6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 7 μg/mL.

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NEK6

Target: NEK6 (NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6))

Alternative Name: NEK6 (NEK6 Products)

Synonyms: 1300007C09Rik antibody, si:ch211-167p9.4 antibody, ATNEK5 antibody, MOE17.17 antibody, NIMA-RELATED KINASE5 antibody, NIMA-related kinase 5 antibody, SID6-1512 antibody, NIMA (never in mitosis gene a)-related expressed kinase 6 antibody, NIMA-related kinase 6 antibody, NIMA related kinase 6 antibody, Serine/Threonine kinase catalytic domain protein antibody, NIMA-related kinase 6 S homeolog antibody, Nek6 antibody, nek6 antibody, NEK6 antibody, NEK5 antibody, nek6.S antibody

Background: Name/Gene ID: NEK6
Subfamily: NEK
Family: Protein Kinase

Synonyms: NEK6, NIMA-related kinase 6, Protein kinase SID6-1512, NimA-related protein kinase 6, SID6-1512

Gene ID: 10783