LPHN2 antibody (N-Term)

Catalog No. ABIN1049025

Product Details anti-LPHN2 Antibody

Target: LPHN2 (Latrophilin 2 (LPHN2))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Cow, Bat, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPHN2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human LPHN2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Platypus (100%) Marmoset, Chicken, Xenopus, Pufferfish (94%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from N-terminal extracellular domain of human LPHN2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Platypus (100%), Marmoset, Chicken, Xenopus, Pufferfish (94%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (7 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LPHN2

Target: LPHN2 (Latrophilin 2 (LPHN2))

Alternative Name: ADGRL2 / LPHN2 (LPHN2 Products)

Synonyms: LPHN2 antibody, CIRL2 antibody, CL2 antibody, LEC1 antibody, LPHH1 antibody, AI450192 antibody, Gm619 antibody, Lec1 antibody, Lphh1 antibody, mKIAA0786 antibody, Cirl-2 antibody, Cirl2 antibody, Cl2ac antibody, LEC2 antibody, adhesion G protein-coupled receptor L2 antibody, ADGRL2 antibody, adgrl2 antibody, LOC100538279 antibody, Adgrl2 antibody

Background: Name/Gene ID: ADGRL2
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRL2, Cl2aa, CIRL2, CL2, Latrophilin 1, Latrophilin-2, Lectomedin-1, Latrophilin 2, Latrophilin homolog 2 (cow), KIAA0786, Beta-latrotoxin receptor, CIRL-2, Latrophilin homolog 1, Latrotoxin receptor 2, Latrotoxin receptor homolog 2, LEC1, LPHH1, LPHN2

Gene ID: 23266