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HRH4 antibody (Cytoplasmic Domain)

HRH4 Reactivity: Human, Monkey IHC, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048964
  • Target See all HRH4 Antibodies
    HRH4 (Histamine Receptor H4 (HRH4))
    Binding Specificity
    • 15
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivity
    • 60
    • 7
    • 2
    • 1
    Human, Monkey
    Host
    • 65
    • 1
    Rabbit
    Clonality
    • 65
    • 1
    Polyclonal
    Conjugate
    • 31
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HRH4 antibody is un-conjugated
    Application
    • 53
    • 31
    • 13
    • 13
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Brand
    IHC-plus™
    Specificity
    Human HRH4 / Histamine H4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset (100%) Bovine, Horse, Rabbit (94%) Gibbon, Mouse, Panda, Dog (89%) Rat, Opossum, Platypus (83%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 18 amino acid peptide from 1st cytoplasmic domain of human HRH4 / Histamine H4 Receptor. Percent identity with other species by BLAST analysis: Human, Monkey, Marmoset (100%), Bovine, Horse, Rabbit (94%), Gibbon, Mouse, Panda, Dog (89%), Rat, Opossum, Platypus (83%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product HRH4 Primary Antibody
  • Application Notes
    Approved: ICC, IF, IHC, IHC-P (12 - 24 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    HRH4 (Histamine Receptor H4 (HRH4))
    Alternative Name
    HRH4 / Histamine H4 Receptor (HRH4 Products)
    Synonyms
    AXOR35 antibody, BG26 antibody, GPCR105 antibody, GPRv53 antibody, H4 antibody, H4R antibody, HH4R antibody, HRH4 antibody, histamine receptor H4 antibody, HRH4 antibody, Hrh4 antibody
    Background
    Name/Gene ID: HRH4
    Subfamily: Histamine
    Family: GPCR

    Synonyms: HRH4, BG26, G-protein coupled receptor 105, GPRv53, H4, HH4R, Histamine H4 receptor, GPCR105, H4R, Pfi-013, SP9144, AXOR35, Gprc105, H4 histamine receptor, Histamine receptor H4
    Gene ID
    59340
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