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HNF4 gamma antibody (Ligand Binding Domain)

HNF4G Reactivity: Human, Cow, Monkey, Pig, Bat IHC (p), IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048954
  • Target See all HNF4 gamma (HNF4G) Antibodies
    HNF4 gamma (HNF4G) (Hepatocyte Nuclear Factor 4 gamma (HNF4G))
    Binding Specificity
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Ligand Binding Domain
    Reactivity
    • 17
    • 11
    • 8
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Cow, Monkey, Pig, Bat
    Host
    • 17
    Rabbit
    Clonality
    • 17
    Polyclonal
    Conjugate
    • 17
    This HNF4 gamma antibody is un-conjugated
    Application
    • 16
    • 3
    • 2
    • 2
    • 2
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
    Brand
    IHC-plus™
    Specificity
    Human HNF4G. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Bat, Pig (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Opossum, Platypus (94%) Horse, Turkey, Chicken (88%) Lizard, Seabass, Pufferfish (82%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from ligand-binding domain of human HNF4G. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Bat, Pig (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Opossum, Platypus (94%), Horse, Turkey, Chicken (88%), Lizard, Seabass, Pufferfish (82%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product HNF4G Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (8 - 16 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    HNF4 gamma (HNF4G) (Hepatocyte Nuclear Factor 4 gamma (HNF4G))
    Alternative Name
    HNF4G / HNF4 gamma (HNF4G Products)
    Synonyms
    HNF4G antibody, hnf4gamma antibody, zgc:153265 antibody, NR2A2 antibody, NR2A3 antibody, hepatocyte nuclear factor 4 gamma antibody, hepatocyte nuclear factor 4, gamma antibody, HNF4G antibody, hnf4g antibody, Hnf4g antibody
    Background
    Name/Gene ID: HNF4G
    Subfamily: NR2 Hepatocyte NF4-like
    Family: NHR

    Synonyms: HNF4G, HNF-4-gamma, HNF4-beta, HNF4 gamma, Hnf4gamma, NR2A3, NR2A2
    Gene ID
    3174
    Pathways
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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