Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

GPR84 antibody (Extracellular Domain)

GPR84 Reactivity: Human, Horse, Monkey IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048884
  • Target See all GPR84 Antibodies
    GPR84 (G Protein-Coupled Receptor 84 (GPR84))
    Binding Specificity
    • 16
    • 7
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reactivity
    • 42
    • 25
    • 7
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Human, Horse, Monkey
    Host
    • 56
    • 3
    Rabbit
    Clonality
    • 57
    • 2
    Polyclonal
    Conjugate
    • 19
    • 6
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GPR84 antibody is un-conjugated
    Application
    • 41
    • 27
    • 21
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human GPR84. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 19 amino acid peptide from 2nd extracellular domain of human GPR84. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Horse (100%), Gibbon (95%), Elephant, Bovine, Rabbit (89%), Mouse, Dog (84%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product GPR84 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    GPR84 (G Protein-Coupled Receptor 84 (GPR84))
    Alternative Name
    GPR84 (GPR84 Products)
    Synonyms
    EX33 antibody, GPCR4 antibody, GPR84 antibody, sb:eu355 antibody, zgc:171802 antibody, G protein-coupled receptor 84 antibody, G protein-coupled receptor 84 S homeolog antibody, GPR84 antibody, Gpr84 antibody, gpr84 antibody, gpr84.S antibody
    Background
    Name/Gene ID: GPR84
    Subfamily: Orphan-A
    Family: GPCR

    Synonyms: GPR84, EX33, GPCR4, G-protein coupled receptor 84, G protein-coupled receptor 84
    Gene ID
    53831
You are here:
Support