GPR45 antibody (Cytoplasmic Domain)

Catalog No. ABIN1048831

Product Details anti-GPR45 Antibody

Target: GPR45 (G Protein-Coupled Receptor 45 (GPR45))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human, Monkey, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR45 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Brand: IHC-plus™

Specificity: Human GPR45. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Horse (100%) Mouse, Panda (94%) Rabbit (88%) Rat, Bat (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human GPR45. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Horse (100%), Mouse, Panda (94%), Rabbit (88%), Rat, Bat (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (42 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR45

Target: GPR45 (G Protein-Coupled Receptor 45 (GPR45))

Alternative Name: GPR45 (GPR45 Products)

Synonyms: GPR45 antibody, MGC97852 antibody, PSP24 antibody, PSP24(ALPHA) antibody, PSP24A antibody, 9230112G11Rik antibody, AW046772 antibody, LPA antibody, lpar-a antibody, xpsp24 antibody, G protein-coupled receptor 45 antibody, G protein-coupled receptor 45 L homeolog antibody, GPR45 antibody, gpr45 antibody, Gpr45 antibody, gpr45.L antibody

Background: Name/Gene ID: GPR45
Subfamily: Orphan-A
Family: GPCR

Synonyms: GPR45, G protein-coupled receptor 45, PSP24(ALPHA), PSP24-1, PSP24, PSP24A, PSP24-alpha

Gene ID: 11250