GPR149 antibody (N-Term)
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- Target See all GPR149 Antibodies
- GPR149 (G Protein-Coupled Receptor 149 (GPR149))
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Binding Specificity
- N-Term
- Reactivity
- Human, Monkey, Bat, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GPR149 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human GPR149. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Pig (100%) Rabbit (93%) Elephant, Bovine (87%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 15 amino acid peptide from N-terminal extracellular domain of human GPR149. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Pig (100%), Rabbit (93%), Elephant, Bovine (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GPR149 Primary Antibody
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- Application Notes
- Approved: IHC, IHC-P (7 - 18 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GPR149 (G Protein-Coupled Receptor 149 (GPR149))
- Alternative Name
- GPR149 / PGR10 (GPR149 Products)
- Synonyms
- GPR149 antibody, IEDA antibody, PGR10 antibody, Ieda antibody, 9630018L10Rik antibody, R35 antibody, G protein-coupled receptor 149 antibody, probable G-protein coupled receptor 149 antibody, GPR149 antibody, LOC100219033 antibody, gpr149 antibody, Gpr149 antibody
- Background
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Name/Gene ID: GPR149
Subfamily: Orphan-U
Family: GPCR
Synonyms: GPR149, PGR10, G protein-coupled receptor 149, IEDA - Gene ID
- 344758
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