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GPR149 antibody (N-Term)

GPR149 Reactivity: Human, Monkey, Bat, Pig IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048730
  • Target See all GPR149 Antibodies
    GPR149 (G Protein-Coupled Receptor 149 (GPR149))
    Binding Specificity
    • 15
    • 8
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    Human, Monkey, Bat, Pig
    Host
    • 41
    Rabbit
    Clonality
    • 41
    Polyclonal
    Conjugate
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GPR149 antibody is un-conjugated
    Application
    • 32
    • 20
    • 13
    • 13
    • 7
    • 3
    • 3
    • 2
    • 2
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human GPR149. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Pig (100%) Rabbit (93%) Elephant, Bovine (87%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 15 amino acid peptide from N-terminal extracellular domain of human GPR149. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Pig (100%), Rabbit (93%), Elephant, Bovine (87%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product GPR149 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (7 - 18 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    GPR149 (G Protein-Coupled Receptor 149 (GPR149))
    Alternative Name
    GPR149 / PGR10 (GPR149 Products)
    Synonyms
    GPR149 antibody, IEDA antibody, PGR10 antibody, Ieda antibody, 9630018L10Rik antibody, R35 antibody, G protein-coupled receptor 149 antibody, probable G-protein coupled receptor 149 antibody, GPR149 antibody, LOC100219033 antibody, gpr149 antibody, Gpr149 antibody
    Background
    Name/Gene ID: GPR149
    Subfamily: Orphan-U
    Family: GPCR

    Synonyms: GPR149, PGR10, G protein-coupled receptor 149, IEDA
    Gene ID
    344758
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