GPR137B antibody (C-Term)
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- Target See all GPR137B products
- GPR137B (G Protein-Coupled Receptor 137B (GPR137B))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Horse, Monkey, Pig, Rabbit
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GPR137B antibody is un-conjugated
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Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Brand
- IHC-plus™
- Specificity
- Human GPR137B / TM7SF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Horse, Rabbit, Pig, Opossum (100%) Hamster, Elephant, Bovine (94%) Goat, Chicken, Lizard (88%) Dog, Bat, Turkey (81%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 16 amino acid peptide from C-terminus of human GPR137B / TM7SF1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Horse, Rabbit, Pig, Opossum (100%), Hamster, Elephant, Bovine (94%), Goat, Chicken, Lizard (88%), Dog, Bat, Turkey (81%).
Type of Immunogen: Synthetic peptide
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- Application Notes
- Approved: ICC, IHC, IHC-P (5 - 12 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GPR137B (G Protein-Coupled Receptor 137B (GPR137B))
- Alternative Name
- GPR137B (GPR137B Products)
- Synonyms
- TM7SF1 antibody, AI788917 antibody, AU016435 antibody, AW546472 antibody, C730041E01 antibody, C80550 antibody, C80741 antibody, D13Abb1e antibody, Tm7sf1 antibody, GPR137B antibody, gpr137ba antibody, tm7sf1a antibody, wu:fi39g02 antibody, zgc:101671 antibody, G protein-coupled receptor 137B antibody, G protein-coupled receptor 137B L homeolog antibody, G protein-coupled receptor 137c antibody, GPR137B antibody, gpr137b.L antibody, Gpr137b antibody, gpr137b antibody, gpr137c antibody
- Background
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Name/Gene ID: GPR137B
Subfamily: Orphan-U
Family: GPCR
Synonyms: GPR137B, TM7SF1 - Gene ID
- 7107
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