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GPR109B antibody (Cytoplasmic Domain)

HCAR3 Reactivity: Human, Dog, Hamster IHC (p), IHC, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048689
  • Target See all GPR109B (HCAR3) Antibodies
    GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))
    Binding Specificity
    • 15
    • 14
    • 9
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivity
    Human, Dog, Hamster
    Host
    • 41
    • 2
    Rabbit
    Clonality
    • 43
    Polyclonal
    Conjugate
    • 19
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GPR109B antibody is un-conjugated
    Application
    • 21
    • 14
    • 13
    • 13
    • 8
    • 4
    • 4
    • 4
    • 3
    • 2
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Brand
    IHC-plus™
    Specificity
    Human GPR109B / HM74. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HCAR2 (100 % ), GPR81 (50 %).
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Dog, Hamster, Panda (100%) Mouse, Horse, Guinea pig (95%) Rat (90%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR109B / HM74. Percent identity with other species by BLAST analysis: Human, Gorilla, Dog, Hamster, Panda (100%), Mouse, Horse, Guinea pig (95%), Rat (90%).

    Type of Immunogen: Synthetic peptide
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    Discover our top product HCAR3 Primary Antibody
  • Application Notes
    Approved: ICC, IHC, IHC-P (19 - 38 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))
    Alternative Name
    HCAR3 / GPR109B / HM74 (HCAR3 Products)
    Synonyms
    GPR109B antibody, HCA3 antibody, HM74 antibody, PUMAG antibody, Puma-g antibody, NIACR2 antibody, hm74 antibody, niacr2 antibody, hydroxycarboxylic acid receptor 3 antibody, HCAR3 antibody, hcar3 antibody
    Background
    Name/Gene ID: HCAR3
    Subfamily: Nicotinic Acid
    Family: GPCR

    Synonyms: HCAR3, GPR109B, GTP-binding protein, NIACR2, HM74, HM74b, Nicotinic acid receptor 2, Putative chemokine receptor, Niacin receptor 2, Puma-g, HCA3, PUMAG
    Gene ID
    8843
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