GPR108 antibody (Cytoplasmic Domain)
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- Target See all GPR108 products
- GPR108 (G Protein-Coupled Receptor 108 (GPR108))
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Binding Specificity
- Cytoplasmic Domain
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GPR108 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human GPR108. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR107 (50 % ).
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset (100%) Monkey, Rat, Hamster, Dog, Horse (94%) Mouse, Panda, Bovine, Pig (89%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 18 amino acid peptide from 1st cytoplasmic domain of human GPR108. Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset (100%), Monkey, Rat, Hamster, Dog, Horse (94%), Mouse, Panda, Bovine, Pig (89%).
Type of Immunogen: Synthetic peptide
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- Application Notes
- Approved: IHC, IHC-P (2.4 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GPR108 (G Protein-Coupled Receptor 108 (GPR108))
- Alternative Name
- GPR108 (GPR108 Products)
- Synonyms
- GPR108 antibody, LUSTR2 antibody, 1810015L19Rik antibody, AA589464 antibody, C79132 antibody, G protein-coupled receptor 108 antibody, GPR108 antibody, Gpr108 antibody
- Background
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Name/Gene ID: GPR108
Subfamily: Orphan-U
Family: GPCR
Synonyms: GPR108, G protein-coupled receptor 108, LUSTR2, Protein GPR108 - Gene ID
- 56927
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