FZD1 antibody (N-Term)

Catalog No. ABIN1048617

Product Details anti-FZD1 Antibody

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Hamster, Horse, Monkey, Rabbit, Bat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FZD1 / Frizzled 1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Shrew, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Armadillo (100%) Elephant (94%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from N-terminal extracellular domain of human FZD1 / Frizzled 1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Shrew, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Armadillo (100%), Elephant (94%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (7 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FZD1

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Alternative Name: FZD1 / Frizzled 1 (Fzd1 Products)

Synonyms: CG17697 antibody, CG3646 antibody, DFZ1 antibody, DFz antibody, DFz1 antibody, Dfz1 antibody, Dm Fz1 antibody, Dmel\\CG17697 antibody, FZ antibody, Frz antibody, Fz antibody, Fz1 antibody, frz antibody, fz1 antibody, fz[[1]] antibody, Fz-1 antibody, cFz-1 antibody, Xfrizzled-1 antibody, frizzled1 antibody, frz-1 antibody, frz1 antibody, fzd1-A antibody, AW227548 antibody, FZ-1 antibody, frizzled antibody, frizzled class receptor 1 antibody, frizzled class receptor 1 L homeolog antibody, fz antibody, FZD1 antibody, Fzd1 antibody, fzd1.L antibody

Background: Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1

Gene ID: 8321

Pathways: WNT Signaling, Asymmetric Protein Localization