ESRRG antibody (C-Term)

Catalog No. ABIN1048587

Product Details anti-ESRRG Antibody

Target: ESRRG (Estrogen-Related Receptor gamma (ESRRG))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Monkey, Chicken, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESRRG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Brand: IHC-plus™

Specificity: Human ESRRG / ERR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%) Bat, Xenopus, Zebrafish (93%) Stickleback, Medaka, Pufferfish (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from C-terminus of human ESRRG / ERR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%), Bat, Xenopus, Zebrafish (93%), Stickleback, Medaka, Pufferfish (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ChrIP, IHC, IHC-P (9 - 17 μg/mL), WB

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ESRRG

Target: ESRRG (Estrogen-Related Receptor gamma (ESRRG))

Alternative Name: ESRRG / ERR gamma (ESRRG Products)

Synonyms: ERR3 antibody, ERRgamma antibody, NR3B3 antibody, ESRRG antibody, USH2A antibody, DKFZp459J0417 antibody, err3 antibody, nr3b3 antibody, LOC100219115 antibody, errg antibody, errgamma antibody, esrrg antibody, errb/g antibody, esrrgl antibody, errbeta/gamma antibody, Errg antibody, mKIAA0832 antibody, estrogen related receptor gamma antibody, estrogen-related receptor gamma antibody, estrogen-related receptor gamma a antibody, estrogen-related receptor gamma b antibody, ESRRG antibody, esrrg antibody, Esrrg antibody, esrrga antibody, esrrgb antibody

Background: Name/Gene ID: ESRRG
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESRRG, ERR Gamma, ERR gamma-2, ERR3, Err-gamma, NR3B3, ERRG2, ERRgamma, KIAA0832

Gene ID: 2104

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway