ESRRB antibody (Ligand Binding Domain)

Catalog No. ABIN1048584

Product Details anti-ESRRB Antibody

Target: ESRRB (Estrogen-Related Receptor beta (ESRRB))

Binding Specificity: Ligand Binding Domain

Reactivity: Human, Horse, Dog, Cow, Rabbit, Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESRRB antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ESRRB. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ESRRG (67 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%) Mouse, Rat, Elephant, Turkey, Chicken, Lizard (93%) Bat (87%) Stickleback, Medaka, Pufferfish, Zebrafish (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from ligand-binding domain of human ESRRB. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%), Mouse, Rat, Elephant, Turkey, Chicken, Lizard (93%), Bat (87%), Stickleback, Medaka, Pufferfish, Zebrafish (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ESRRB

Target: ESRRB (Estrogen-Related Receptor beta (ESRRB))

Alternative Name: ESRRB / ERR beta (ESRRB Products)

Synonyms: errb antibody, errbeta antibody, ESRRB antibody, Err2 antibody, Errb antibody, Estrrb antibody, Nr3b2 antibody, DFNB35 antibody, ERR2 antibody, ERRb antibody, ESRL2 antibody, NR3B2 antibody, estrogen-related receptor beta antibody, estrogen related receptor beta antibody, estrogen related receptor, beta antibody, esrrb antibody, ESRRB antibody, Esrrb antibody

Background: Name/Gene ID: ESRRB
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESRRB, DFNB35, ERRb, ERRbeta-2, ESRL2, Estrogen receptor-like 2, ERR-beta, ERRB2, ERRbeta, NR3B2, Nuclear receptor ERRB2, Orphan nuclear receptor, ERR beta-2, ERR2, Estrogen-related receptor 2, Estrogen-related receptor beta, Steroid hormone receptor ERR2

Gene ID: 2103

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance