ESRRA antibody (Internal Region)

Catalog No. ABIN1048580

Product Details anti-ESRRA Antibody

Target: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Pig, Monkey, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESRRA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Brand: IHC-plus™

Specificity: Human ESRRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Pig (100%) Gibbon (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from internal region of human ESRRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Pig (100%), Gibbon (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ChrIP, IHC, IHC-P (8 - 24 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ESRRA

Target: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Alternative Name: ESRRA / ERR alpha (ESRRA Products)

Synonyms: err1 antibody, erra antibody, esrral antibody, erralpha antibody, nr3b1 antibody, MGC80854 antibody, ESRRA antibody, esrl1 antibody, LOC100125507 antibody, ERRalpha antibody, Err1 antibody, Estrra antibody, Nr3b1 antibody, Errra antibody, ERR1 antibody, ERRa antibody, ESRL1 antibody, NR3B1 antibody, ESTRRA antibody, estrogen-related receptor alpha antibody, estrogen related receptor alpha L homeolog antibody, estrogen related receptor alpha antibody, catsper channel auxiliary subunit zeta antibody, estrogen related receptor, alpha antibody, esrra antibody, esrra.L antibody, ESRRA antibody, LOC100125507 antibody, CATSPERZ antibody, Esrra antibody

Background: Name/Gene ID: ESRRA
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESRRA, ERRa, ERR1, HERR1, NR3B1, Steroid hormone receptor ERR1, ERR-alpha, ERRalpha, ESRL1, Estrogen receptor-like 1

Gene ID: 2101

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha