DPP6 antibody (Extracellular Domain)

Catalog No. ABIN1048509

Product Details anti-DPP6 Antibody

Target: DPP6 (Dipeptidyl-Peptidase 6 (DPP6))

Binding Specificity: Extracellular Domain

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPP6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DPP6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Xenopus (100%) Pig, Opossum, Platypus, Lizard (94%) Turkey, Chicken, Zebrafish (89%) Stickleback, Pufferfish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from extracellular domain of human DPP6. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Xenopus (100%), Pig, Opossum, Platypus, Lizard (94%), Turkey, Chicken, Zebrafish (89%), Stickleback, Pufferfish (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DPP6

Target: DPP6 (Dipeptidyl-Peptidase 6 (DPP6))

Alternative Name: DPP6 / Dipeptidylpeptidase 6 (DPP6 Products)

Synonyms: dppx antibody, DPPX antibody, VF2 antibody, DPP VI antibody, dpp6 antibody, si:ch211-198f16.1 antibody, B930011P16Rik antibody, D5Buc3 antibody, D5Buc4 antibody, D5Buc5 antibody, Dpp-6 antibody, Gm1377 antibody, In(5)6H-p antibody, Peplb antibody, Rw antibody, dipeptidyl peptidase like 6 antibody, dipeptidyl-peptidase 6 antibody, dipeptidyl-peptidase 6b antibody, dipeptidylpeptidase 6 antibody, DPP6 antibody, dpp6 antibody, BCAN_A2221 antibody, BSUIS_A2016 antibody, Bsph_2289 antibody, BMEA_A2239 antibody, dpp6b antibody, Dpp6 antibody

Background: Name/Gene ID: DPP6
Subfamily: Serine S9B
Family: Protease

Synonyms: DPP6, Dipeptidylpeptidase VI, Dipeptidyl peptidase 6, Dipeptidyl peptidase VI, Dipeptidyl-peptidase 6, Dipeptidylpeptidase 6, DPP VI, DPPX, VF2, Dipeptidyl aminopeptidase-like protein 6

Gene ID: 1804