DKK1 antibody (Internal Region)

Catalog No. ABIN1048502

Product Details anti-DKK1 Antibody

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Binding Specificity: Internal Region

Reactivity: Human, Horse, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DKK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Bat, Horse, Pig (100%) Galago, Sheep, Goat, Elephant, Bovine, Rabbit, Guinea pig (94%) Hamster, Panda, Dog (88%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from internal region of human DKK1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Bat, Horse, Pig (100%), Galago, Sheep, Goat, Elephant, Bovine, Rabbit, Guinea pig (94%), Hamster, Panda, Dog (88%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1 (DKK1 Products)

Synonyms: DKK-1 antibody, SK antibody, AI414495 antibody, SGY antibody, SGY-1 antibody, SGY1 antibody, Soggy antibody, mSgy antibody, dkk1 antibody, dkk antibody, dkk-1 antibody, xdkk1 antibody, Xdkk-1 antibody, Dickkopf1 antibody, dickkopf-1 antibody, mdkk-1 antibody, dkk1-A antibody, dickkopf WNT signaling pathway inhibitor 1 antibody, dickkopf like acrosomal protein 1 antibody, dickkopf-related protein 1 antibody, dickkopf-like 1 antibody, dickkopf WNT signaling pathway inhibitor 1b antibody, dickkopf protein antibody, dickkopf WNT signaling pathway inhibitor 1 S homeolog antibody, DKK1 antibody, Dkk1 antibody, DKKL1 antibody, LOC100228789 antibody, Dkkl1 antibody, dkk1b antibody, dkk1 antibody, dickkopf antibody, dkk1.S antibody

Background: Name/Gene ID: DKK1

Synonyms: DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK

Gene ID: 22943

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation