CCRL1 antibody (Cytoplasmic Domain)

Catalog No. ABIN1048408

Product Details anti-CCRL1 Antibody

Target: CCRL1 (Chemokine (C-C Motif) Receptor-Like 1 (CCRL1))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human, Mouse, Rat, Rabbit, Horse, Dog, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCRL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CCRL1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CCR6 (63 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig (100%) Marmoset, Bovine, Hamster, Opossum (94%) Turkey, Chicken, Xenopus (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human CCRL1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig (100%), Marmoset, Bovine, Hamster, Opossum (94%), Turkey, Chicken, Xenopus (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (24 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CCRL1

Target: CCRL1 (Chemokine (C-C Motif) Receptor-Like 1 (CCRL1))

Alternative Name: ACKR4 / CCRL1 / CCR11 (CCRL1 Products)

Synonyms: CC-CKR-11 antibody, CCBP2 antibody, CCR-11 antibody, CCR10 antibody, CCR11 antibody, CCRL1 antibody, CCX CKR antibody, CCX-CKR antibody, CKR-11 antibody, PPR1 antibody, VSHK1 antibody, A630091E18Rik antibody, CCX-CKR1 antibody, Ccrl1 antibody, Cmkbrl1 antibody, atypical chemokine receptor 4 antibody, chemokine (C-C motif) receptor-like 1 antibody, Chemokine receptor-like 1 antibody, chemerin chemokine-like receptor 1 antibody, ACKR4 antibody, Ackr4 antibody, CCRL1 antibody, cml1 antibody, CMKLR1 antibody

Background: Name/Gene ID: ACKR4
Subfamily: Chemokine
Family: GPCR

Synonyms: ACKR4, C-C CKR-11, CC-CKR-11, CC chemokine receptor-like 1, CCR11, CCX CKR, C-C chemokine receptor type 11, CKR-11, CCRL1, VSHK1, CCR-11, CCX-CKR, PPR1

Gene ID: 51554