Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CALCRL antibody (Cytoplasmic Domain)

CALCRL Reactivity: Human, Rat, Cow, Dog, Horse, Rabbit, Goat, Monkey, Hamster, Pig, Xenopus laevis IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048377
  • Target See all CALCRL Antibodies
    CALCRL (Calcitonin Receptor-Like (CALCRL))
    Binding Specificity
    • 7
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivity
    Human, Rat, Cow, Dog, Horse, Rabbit, Goat, Monkey, Hamster, Pig, Xenopus laevis
    Host
    • 37
    • 1
    Rabbit
    Clonality
    • 38
    Polyclonal
    Conjugate
    • 23
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CALCRL antibody is un-conjugated
    Application
    • 21
    • 14
    • 6
    • 3
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human CALCRL. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CALCR (56 % ).
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Goat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus (100%) Mouse, Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (94%) Turkey, Chicken (81%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CALCRL. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Goat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus (100%), Mouse, Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (94%), Turkey, Chicken (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product CALCRL Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (34 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Zheng, Nixdorf-Bergweiler, van Brederode, Alzheimer, Messlinger: "Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices." in: International journal of molecular sciences, Vol. 22, Issue 7, (2021) (PubMed).

  • Target
    CALCRL (Calcitonin Receptor-Like (CALCRL))
    Alternative Name
    CALCRL / CGRP Receptor (CALCRL Products)
    Synonyms
    CALCRL antibody, CGRPR antibody, CRLR antibody, AV071593 antibody, CLR antibody, Crlr antibody, RATCRLR antibody, RNCLR antibody, calcitonin receptor like receptor antibody, calcitonin receptor-like antibody, calcitonin receptor like receptor L homeolog antibody, CALCRL antibody, LOC100168906 antibody, calcrl antibody, Calcrl antibody, calcrl.L antibody
    Background
    Name/Gene ID: CALCRL
    Subfamily: Adrenomedullin
    Family: GPCR

    Synonyms: CALCRL, CGRP type 1 receptor, Cgrp1 receptor, CRLR, Calcitonin receptor-like, CGRP receptor, CGRPR
    Gene ID
    10203
    Pathways
    cAMP Metabolic Process
You are here:
Support