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Cell Adhesion Molecule 4 antibody (Internal Region)

CADM4 Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Monkey, Pig, Bat IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048370
  • Target See all Cell Adhesion Molecule 4 (CADM4) Antibodies
    Cell Adhesion Molecule 4 (CADM4)
    Binding Specificity
    • 15
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Monkey, Pig, Bat
    Host
    • 34
    • 3
    • 2
    • 1
    Rabbit
    Clonality
    • 37
    • 3
    Polyclonal
    Conjugate
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Cell Adhesion Molecule 4 antibody is un-conjugated
    Application
    • 32
    • 13
    • 13
    • 12
    • 8
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human CADM4 / NECL-4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 14 amino acid peptide from internal region of human CADM4 / NECL-4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product CADM4 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (10 - 15 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    Cell Adhesion Molecule 4 (CADM4)
    Alternative Name
    IGSF4C / CADM4 (CADM4 Products)
    Synonyms
    CADM4 antibody, IGSF4C antibody, zgc:101704 antibody, NECL4 antibody, Necl-4 antibody, TSLL2 antibody, synCAM4 antibody, Igdf4c antibody, Igsf4c antibody, Tsll2 antibody, Upar antibody, cell adhesion molecule 4 antibody, cell adhesion molecule 4 L homeolog antibody, CADM4 antibody, cadm4 antibody, Cadm4 antibody, cadm4.L antibody
    Background
    Name/Gene ID: CADM4

    Synonyms: CADM4, Cell adhesion molecule 4, Nectin-like 4, Nectin-like protein 4, Necl-4, NECL4, IGSF4C, TSLC1-like 2, SynCAM4, TSLC1-like protein 2, TSLL2
    Gene ID
    199731
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