CADM2 antibody (Internal Region)

Catalog No. ABIN1048369

Product Details anti-CADM2 Antibody

Target: CADM2 (Cell Adhesion Molecule 2 (CADM2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Bat, Horse, Monkey, Rabbit, Hamster, Guinea Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CADM2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CADM2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Guinea pig, Zebra finch, Platypus, Xenopus (100%) Turkey, Chicken (94%) Zebrafish (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from internal region of human CADM2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Guinea pig, Zebra finch, Platypus, Xenopus (100%), Turkey, Chicken (94%), Zebrafish (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL)

Usage: Antigen retrieval with HIER and sodium citrate at  pH 6.0 is required for Immunohistochemistry using formalin-fixed paraffin-embedded human tissues.

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CADM2

Target: CADM2 (Cell Adhesion Molecule 2 (CADM2))

Alternative Name: CADM2 (CADM2 Products)

Synonyms: 2900078E11Rik antibody, 9330131D06 antibody, A830029E02Rik antibody, Igdf4d antibody, Igsf4d antibody, NECL3 antibody, SynCAM 2 antibody, SynCAM2 antibody, Necl-3 antibody, IGSF4D antibody, synCAM2 antibody, igsf4d antibody, zgc:66339 antibody, cell adhesion molecule 2 antibody, cell adhesion molecule 2a antibody, CADM2 antibody, Cadm2 antibody, cadm2a antibody

Background: Name/Gene ID: CADM2

Synonyms: CADM2, Cell adhesion molecule 2, IGSF4D, Necl-3, Nectin-like 3, SynCAM2, Nectin-like protein 3, SynCAM 2, NECL3

Gene ID: 253559

Pathways: Cell-Cell Junction Organization