Bestrophin 2 antibody (Internal Region)

Catalog No. ABIN1048350

Product Details anti-Bestrophin 2 Antibody

Target: Bestrophin 2 (BEST2)

Binding Specificity: Internal Region

Reactivity: Human, Monkey, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bestrophin 2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BEST2 / Bestrophin-2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except BEST1 (47 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog (100%) Mouse, Hamster, Panda, Bovine (93%) Opossum (87%) Elephant, Pig (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from internal region of human BEST2 / Bestrophin-2. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog (100%), Mouse, Hamster, Panda, Bovine (93%), Opossum (87%), Elephant, Pig (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Bestrophin 2

Target: Bestrophin 2 (BEST2)

Alternative Name: BEST2 / Bestrophin-2 (BEST2 Products)

Synonyms: VMD2L1 antibody, CG10173 antibody, Dmel\\CG10173 antibody, dbest2 antibody, Vmd2l1 antibody, best2 antibody, vmd2l1 antibody, MGC53680 antibody, MGC132361 antibody, Best2 antibody, GB16861 antibody, si:dkey-121b10.4 antibody, MGC75942 antibody, best2-a antibody, best2-b antibody, variant 2 antibody, bestrophin 2 antibody, Bestrophin 2 antibody, bestrophin 2 L homeolog antibody, uncharacterized LOC408898 antibody, bestrophin 2 S homeolog antibody, BEST2 antibody, Best2 antibody, best2.L antibody, LOC408898 antibody, best2 antibody, best2.S antibody

Background: Name/Gene ID: BEST2
Subfamily: Bestrophin
Family: Ion channel

Synonyms: BEST2, Bestrophin-2, Bestrophin 2, Hbest2, VMD2-like gene 1, VMD2L1

Gene ID: 54831