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BDKRB1 antibody (Cytoplasmic Domain)

BDKRB1 Reactivity: Human WB, IHC (p), IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048348
  • Target See all BDKRB1 Antibodies
    BDKRB1 (Bradykinin Receptor B1 (BDKRB1))
    Binding Specificity
    • 16
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivity
    • 61
    • 22
    • 22
    • 4
    • 1
    • 1
    Human
    Host
    • 58
    • 3
    • 1
    Rabbit
    Clonality
    • 61
    • 1
    Polyclonal
    Conjugate
    • 28
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BDKRB1 antibody is un-conjugated
    Application
    • 48
    • 19
    • 18
    • 13
    • 13
    • 11
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
    Brand
    IHC-plus™
    Specificity
    Human BDKRB1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Monkey (94%) Horse (81%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human BDKRB1. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Monkey (94%), Horse (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product BDKRB1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB

    Not recommended for: ELISA
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    BDKRB1 (Bradykinin Receptor B1 (BDKRB1))
    Alternative Name
    B1R / BDKRB1 (BDKRB1 Products)
    Synonyms
    bdkrb2 antibody, LOC100230525 antibody, B1BKR antibody, B1R antibody, BKB1R antibody, BKR1 antibody, BRADYB1 antibody, BKR antibody, Bdkrb antibody, b1bkr antibody, bradykinin receptor B1 antibody, bradykinin receptor, beta 1 antibody, bdkrb1 antibody, BDKRB1 antibody, Bdkrb1 antibody
    Background
    Name/Gene ID: BDKRB1
    Subfamily: Bradykinin
    Family: GPCR

    Synonyms: BDKRB1, BKB1R, Bradykinin receptor 1, B1BKR, B1R, Bk b1 receptor, BK-1 receptor, BRADYB1, Bradykinin receptor B1, Kinin b1 receptor, B1 bradykinin receptor, BKR1, Bradykinin B1 receptor
    Gene ID
    623
    Pathways
    ACE Inhibitor Pathway
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