Serotonin Receptor 2B antibody (Extracellular Domain)
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- Target See all Serotonin Receptor 2B (HTR2B) Antibodies
- Serotonin Receptor 2B (HTR2B)
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Binding Specificity
- Extracellular Domain
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Reactivity
- Human, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Serotonin Receptor 2B antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human 5HT2B Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Bovine, Bat (93%) Mouse, Hamster, Panda, Horse, Rabbit, Pig (80%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 15 amino acid peptide from 2nd extracellular domain of human 5HT2B Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Bovine, Bat (93%), Mouse, Hamster, Panda, Horse, Rabbit, Pig (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HTR2B Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (4 - 6 μg/mL)
Not recommended for: ICC - Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Serotonin Receptor 2B (HTR2B)
- Alternative Name
- HTR1B / 5-HT2B Receptor (HTR2B Products)
- Synonyms
- 5-HT2B antibody, 5HT2B antibody, 5htr2b antibody, HTR2B antibody, 5ht2b antibody, zgc:194094 antibody, zgc:194119 antibody, AJ012488 antibody, AV377389 antibody, 5-HT(2B) antibody, 5-hydroxytryptamine receptor 2B antibody, 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled antibody, 5-hydroxytryptamine (serotonin) receptor 2B antibody, HTR2B antibody, htr2b antibody, Htr2b antibody
- Background
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Name/Gene ID: HTR2B
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR2B, 5-HT 2B receptor, 5HT2B Receptor, 5-HT(2B), 5-HT-2B, 5-HT2b receptor, 5-HT2B, Serotonin 2b receptor, Serotonin 5-HT-2b receptor, Serotonin receptor 2B - Gene ID
- 3357
- Pathways
- JAK-STAT Signaling, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Carbohydrate Metabolic Process
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