5HT1B Receptor antibody (Internal Region)

Catalog No. ABIN1048254

Product Details anti-5HT1B Receptor Antibody

Target: 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Binding Specificity: Internal Region

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 5HT1B Receptor antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human 5HT1B Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Bat, Horse, Pig (94%) Hamster, Panda, Bovine, Dog, Cat, Rabbit, Guinea pig (88%) Elephant, Opossum (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from internal region of human 5HT1B Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Bat, Horse, Pig (94%), Hamster, Panda, Bovine, Dog, Cat, Rabbit, Guinea pig (88%), Elephant, Opossum (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for 5HT1B Receptor

Target: 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Alternative Name: HTR1B / 5-HT1B Receptor (HTR1B Products)

Synonyms: 5-HT1B antibody, 5-HT1DB antibody, HTR1D2 antibody, HTR1DB antibody, S12 antibody, 5-HT1Db antibody, 5-HT1-LIKE antibody, HTR1B antibody, 5-HT-dro2B antibody, 5-HT1BDro antibody, 5-HT[1B]Dro antibody, 5-HT[[1BDro]] antibody, 5-HT[[1B]] antibody, 5-HT[[1B]]DRO antibody, 5-HT[[1B]]Dro antibody, 5-HT[[dro2B]] antibody, 5HT-R2B antibody, 5HT-dro2B antibody, 5HT-dro[[2B]] antibody, 5HT1B antibody, 5htdro2b antibody, CG15113 antibody, CG30126 antibody, DRO2B antibody, Dm5HTdro2B antibody, Dmel\\CG15113 antibody, Dmel_CG30126 antibody, d5-HT1B antibody, 5-HT-1B antibody, zgc:194666 antibody, zgc:194690 antibody, 5-hydroxytryptamine receptor 1B antibody, 5-hydroxytryptamine (serotonin) receptor 1B antibody, HTR1B antibody, Htr1b antibody, 5-HT1B antibody, htr1b antibody

Background: Name/Gene ID: HTR1B
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1b receptor, 5-HT1DB, 5HT1B Receptor, HTR1DB, Serotonin 5-HT-1b receptor, Serotonin receptor 1B, Serotonin 1b receptor, HTR1D2, Serotonin 1D beta receptor

Gene ID: 3351

Pathways: JAK-STAT Signaling, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteins