NFYB antibody (N-Term)

Catalog No. ABIN104771

Product Details anti-NFYB Antibody

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Gel Shift (GS)

Purpose: NF-Y Antibody

Cross-Reactivity (Details): A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.

Characteristics: Synonyms: rabbit anti-NF-Y Antibody, Nuclear transcription factor Y subunit B

Purification: This product was prepared from monospecific antiserum by a multi-step procedure which includes delipidation, salt fractionation and ion exchange chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Gel Shift Dilution: 2.0 to 4.0 μL

Application Note: This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific).  Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y.  This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 μL per assay.  This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#611-1302) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate (#ABTS-100).  A dilution of 1:4000 is suggested from this experiment.  Minimal reactivity was observed by ELISA against the A subunit of NF-Y. 

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-NFYB antibody (ABIN104771)

Manni, Caretti, Artuso, Gurtner, Emiliozzi, Sacchi, Mantovani, Piaggio: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, (2008) (PubMed).

Target Details for NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Alternative Name: NFYB (NFYB Products)

Background: Background: Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Gene ID: 4801

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha