NFYB antibody (N-Term)

Catalog No. ABIN104771

Product Details anti-NFYB Antibody

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYB antibody is un-conjugated

Application: ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: NF-Y (B subunit) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific).  Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y.  This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 µl per assay.  This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate.  A dilution of 1:4000 is suggested from this experiment.  Minimal reactivity was observed by ELISA against the A subunit of NF-Y. 

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-NFYB antibody (ABIN104771)

Manni, Caretti, Artuso, Gurtner, Emiliozzi, Sacchi, Mantovani, Piaggio: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, (2008) (PubMed).

Target Details for NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Abstract: NFYB Products

Synonyms: CBF-A antibody, CBF-B antibody, HAP3 antibody, NF-YB antibody, NFYB antibody, nfyb antibody, zgc:110533 antibody, zgc:110552 antibody, hap3 antibody, cbf-a antibody, cbf-b antibody, CBFB antibody, Nfya antibody, AA985999 antibody, Cbf-A antibody, nuclear transcription factor Y subunit beta antibody, nuclear transcription factor Y subunit beta L homeolog antibody, nuclear transcription factor Y, beta b antibody, nuclear transcription factor Y, beta a antibody, nuclear transcription factor-Y beta antibody, NFYB antibody, nfyb.L antibody, nfybb antibody, nfyba antibody, nfyb antibody, Nfyb antibody

Background: Synonyms: Nuclear transcription factor Y subunit B

Gene ID: 4801

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha