Nestin antibody (AA 1484-1500)

Catalog No. ABIN104564

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: AA 1484-1500

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
Immunogentype:Recombinant

Isotype: IgG

Application Details

Application Notes: Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC) and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Nestin antibody (ABIN104564)

Mokrý, Cízková, Filip, Ehrmann, Osterreicher, Kolár, English: "Nestin expression by newly formed human blood vessels." in: Stem cells and development, Vol. 13, Issue 6, pp. 658-64, (2005) (PubMed).

Aihara, Sugawara, Torii, Hosaka, Kurihara, Saito, Takeuchi: "Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 12, pp. 1581-92, (2005) (PubMed).

Yaworsky, Kappen: "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene." in: Developmental biology, Vol. 205, Issue 2, pp. 309-21, (1999) (PubMed).

Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." in: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).

Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Synonyms: paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

Background: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985).  Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube.  Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells.  Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.  Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Synonyms: nestin, ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody

Gene ID: 10763, 38176300

UniProt: P48681