Myosin antibody (pSer19, pSer20)

Catalog No. ABIN104491

Product Details anti-Myosin Antibody

Target: Myosin

Binding Specificity: pSer19, pSer20

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myosin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: This phospho specific polyclonal antibody was tested by ELISA, immunohistochemistry and immunoblotting.   Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes.  The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.  Although not tested, this antibody is likely functional by immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.92 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Myosin antibody (ABIN104491)

Harbom, Rudisill, Michel, Litwa, Beenhakker, McConnell: "The effect of rho kinase inhibition on morphological and electrophysiological maturity in iPSC-derived neurons." in: Cell and tissue research, Vol. 375, Issue 3, pp. 641-654, (2019) (PubMed).

Salomon, Gaston, Magescas, Duvauchelle, Canioni, Sengmanivong, Mayeux, Michaux, Campeotto, Lemale, Viala, Poirier, Minc, Schmitz, Brousse, Ladoux, Goulet, Delacour: "Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity." in: Nature communications, Vol. 8, pp. 13998, (2018) (PubMed).

Panousopoulou, Hobbs, Mason, Green, Formstone: "Epiboly generates the epidermal basal monolayer and spreads the nascent mammalian skin to enclose the embryonic body." in: Journal of cell science, Vol. 129, Issue 9, pp. 1915-27, (2017) (PubMed).

Logue, Cartagena-Rivera, Baird, Davidson, Chadwick, Waterman: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." in: eLife, Vol. 4, pp. e08314, (2016) (PubMed).

Newell-Litwa, Badoual, Asmussen, Patel, Whitmore, Horwitz: "ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity." in: The Journal of cell biology, Vol. 210, Issue 2, pp. 225-42, (2016) (PubMed).

Roland, Ricobaraza, Carrel, Jordan, Rico, Simon, Humbert-Claude, Ferrier, McFadden, Scheuring, Lenkei: "Cannabinoid-induced actomyosin contractility shapes neuronal morphology and growth." in: eLife, Vol. 3, pp. e03159, (2015) (PubMed).

Solanes, Heuzé, Maurin, Bretou, Lautenschlaeger, Maiuri, Terriac, Thoulouze, Launay, Piel, Vargas, Lennon-Duménil: "Space exploration by dendritic cells requires maintenance of myosin II activity by IP3 receptor 1." in: The EMBO journal, Vol. 34, Issue 6, pp. 798-810, (2015) (PubMed).

Fabbiano, Menacho-Márquez, Sevilla, Albarrán-Juárez, Zheng, Offermanns, Montero, Bustelo: "Genetic dissection of the vav2-rac1 signaling axis in vascular smooth muscle cells." in: Molecular and cellular biology, Vol. 34, Issue 24, pp. 4404-19, (2015) (PubMed).

Edelson, Brautigan: "The Discodermia calyx toxin calyculin a enhances cyclin D1 phosphorylation and degradation, and arrests cell cycle progression in human breast cancer cells." in: Toxins, Vol. 3, Issue 1, pp. 105-19, (2013) (PubMed).

Target Details for Myosin

Target: Myosin

Abstract: Myosin Products

Synonyms: CG3201 antibody, DmMLC_C antibody, Dmel\\CG3201 antibody, MyoII antibody, mlc-c antibody, nmELC antibody, MYOSIN antibody, myosin 1 antibody, DFNA4 antibody, DFNA4A antibody, MHC16 antibody, MYH17 antibody, NMHC II-C antibody, NMHC-II-C antibody, PNMHH antibody, myosin antibody, MYH1 antibody, MYH2 antibody, MYH3 antibody, MYH4 antibody, MYH8 antibody, adult antibody, embryonic antibody, 2400004E04Rik antibody, II-C antibody, NHMCII antibody, DROMHC antibody, Dm nmII antibody, DmnmII antibody, Dronm-MII antibody, E(br) antibody, MHC antibody, Mhc-c antibody, Mhc-c[1] antibody, Myo antibody, Myo II antibody, NMM antibody, Zip antibody, anon-WO0140519.37 antibody, l(2)02957 antibody, nmMHC antibody, nmy-2 antibody, zpr antibody, DmelCG15792 antibody, CG15792 antibody, Myosin light chain cytoplasmic antibody, hypothetical protein antibody, myosin 1 antibody, myosin heavy chain 9 antibody, myosin heavy chain 14 antibody, myosin, heavy chain 1B, skeletal muscle antibody, myosin, heavy polypeptide 14 antibody, zipper antibody, Mlc-c antibody, T22C1.6 antibody, ATM1 antibody, MYH9 antibody, MYH14 antibody, Myh14 antibody, MYH1B antibody, zip antibody

Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  This antibody is specific for the phosphorylated form of myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site.  Human and mouse have almost identical sequences.  In human the phosphorylation site is pS19, while in mouse the site maps to pS20.
Synonyms: Myosin regulatory light chain 12A Alternative name(s): Myosin regulatory light chain MRLC3 Myosin regulatory light chain 2, nonsarcomeric Myosin RLC MLC-2B

Gene ID: 10627

UniProt: P19105