RFP antibody

Catalog No. ABIN1043926

Product Details anti-RFP Antibody

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Chicken

Clonality: Polyclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Anti-RFP is designed to detect recombinant RFP.

Specificity: Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry.

Sterility: Sterile filtered

Immunogen: Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes: Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Expiry Date: 3 months

References for anti-RFP antibody (ABIN1043926)

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Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP (RFP Products)

Background: Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.

Gene ID: 55976617

UniProt: Q9U6Y8