TUBA1B antibody (AA 425-451) (Biotin)

Catalog No. ABIN1043919

Supplier Product No.: 600-406-880

Product Details anti-TUBA1B Antibody (Biotin)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Binding Specificity: AA 425-451

Reactivity: Human, Mouse, Rat, Chicken, Cow, Goat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBA1B antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-406-880

Supplier: Rockland

Purpose: Alpha-Tubulin Antibody Biotin Conjugated

Cross-Reactivity (Details): Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.

Characteristics: Synonyms: rabbit anti-alpha-Tubulin Antibody biotin conjugation, biotin conjugated rabbit anti-α-tubulin antibody, biotin conjugated rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody biotin conjugation, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

Purification: The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Optional[Immunogen]: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000-1:100,000

IF Microscopy Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.61 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot Control Antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Tubulin Antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-TUBA1B antibody (Biotin) (ABIN1043919)

Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, (1985) (PubMed).

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: TUBA1B (TUBA1B Products)

Background: Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase