GST antibody (FITC)

Catalog No. ABIN1043914

Supplier Product No.: 600-402-200

Product Details anti-GST Antibody (FITC)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GST antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.: 600-402-200

Supplier: Rockland

Purpose: GST Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Characteristics: Synonyms: rabbit anti-GST antibody fluorescein conjugation, FITC conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Purification: FITC conjugated GST Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 3.0

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: FITC conjugated GST Antibody has been tested by western blot and is ideal for microscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:60,000

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store FITC conjugated GST Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST (GST Products)

Background: Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.